Characterisation of Bovine Amniotic Membrane with Hydroxyapatite Bio-Composite

The fabrication of bio-composite-derived bovine amniotic membrane (BAM) with hydroxyapatite (HAp) is an approach to combining organic and inorganic bio-material improve the properties both materials. This research aims combine, fabricate characterise bio-composite BAM–HA. combination made from BAM HAp in a ratio 30:70, 35:65, 40:60. Dried immersed saline then blended until it forms slurry jelly-like consistency. At this stage, added so that can bind BAM. After mixture homogeneous, freeze-drying process carried out. fabrication, all bio-composites were characterised using Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) porosity analysis, biological activity was conducted fibroblasts. has functional groups amides I, II, III, A, B, OH, CO32− PO43− according results FTIR. XRD analysis showed presence HAP crystals. group crystal indicate these two materials are bound. An SEM examination revealed variety porous patterns on surface area. at 35:65 higher mean pore size 155.625 µm 89.23% maintain fibroblast viability 95.14%. In conclusion, study successfully combined bio-materials HAp, which have potential synergistic effects soft hard tissue regeneration. good characteristics non-toxic.